Morphological and molecular characterization of Pratylenchus thornei Sher & Allen, 1953 (Rhabditida: Pratylenchidae) from Iraq

Document Type : Original Article

Authors

Department of Plant Protection, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz, Iran

Abstract

During an investigation on the biodiversity of plant-parasitic nematodes in Misan province (southeastern Iraq), two populations of Pratylenchus thornei were isolated from the rhizosphere of faba bean. The morphological and morphometric data were provided for the recovered populations. Both populations were similar to each other, but they were somewhat different in terms of the tail shape and body length. The tail in the Alkahla population was subcylindrical, tail terminus truncated and sometimes with a small projection. The tail in the Ali-Algharbi population was conical with an almost round to widely rounded terminus. The morphological and morphometric characters of both populations agree with the type population of the species and some other populations reported from different areas. Molecular phylogenetic analysis of the Iraqi populations of P. thornei using the large subunit ribosomal RNA gene (LSU rDNA D2-D3) and internal transcribed spacer (ITS rDNA) sequences using Bayesian inference (BI), showed they form maximally supported clades with other sequences of the species. The present study is the first to report morphological and molecular data of P. thornei from Iraq.

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