نوع مقاله : مقاله پژوهشی
نویسندگان
1 1- گروه گیاهپزشکی، دانشکده کشاورزی و منابع طبیعی، دانشگاه تهران، کرج، ایران
2 گروه گیاهپزشکی، دانشکده کشاورزی و منابع طبیعی، دانشگاه تهران، کرج، ایران
3 گروه گیاهپزشکی، دانشگاه فردوسی مشهد، مشهد، ایران
چکیده
کلیدواژهها
عنوان مقاله [English]
نویسندگان [English]
During a 2021 survey on insect-associated nematodes in forests of Guilan province, northern Iran, samples of soil, decaying wood, and insects were collected and examined. Analyses of morphological and morphometric characteristics of the isolated nematodes revealed that the populations belong to the genera Metarhabditis and Diploscapter. They were identified as Metarhabditis blumi and Diploscapter coronatus, respectively. To support this identification, DNA sequences were used for Nblast analysis and the phylogenetic trees reconstructed based on the sequences of the internal transcribed spacer (ITS) region of rRNA gene (for M. blumi) and the 18S rRNA gene (for D. coronatus.). Since the sequences of type populations were not available in public databases, comparisons were made with other identified and reliable populations of the same species, which confirmed the morphological identifications. This study represents the first record of M. blumi and the first report of the male of D. coronatus from Iran.
کلیدواژهها [English]