نوع مقاله : مقاله پژوهشی
نویسندگان
بخش گیاه پزشکی، دانشکده کشاورزی، دانشگاه شیراز، شیراز، ایران
چکیده
کلیدواژهها
عنوان مقاله [English]
نویسندگان [English]
The white-tip nematode, Aphelenchoides besseyi is an important plant-parasitic nematode that can also feed on fungi. In this study the effect of feeding on four soil-inhabiting fungi on the morphological and morphometric characteristics of A. besseyi was investigated. For this purpose, the crown tissue of an infected rice plant was cultured on potato dextrose agar medium. After 48 hours, A. besseyi and Fusarium sp. were appeared on the medium. The morphological and morphometric indices of the nematodes extracted from the media, as the initial population were measured using a light microscope. The nematodes were grown on the fungi Curvularia sp., Bipolaris sp., Urocladium sp. and Fusarium sp. at 25-27°C for three months. Later on, the nematodes were extracted by the tray method and morphometrical measurements performed. Isolated nematodes from each fungus were kept on the other fungi for six months and their characteristics studied. The results showed that A. besseyi was able to feed on the fungi but they were not suitable food sources for the nematode. Except the distance from anterior end to the end of pharyngeal glands, the means of other measurements of the populations fed with the fungi were decreased compared to the initial population. Body length, body width at different parts, the distance between anterior end to the center of median bulb, the end of pharyngeal glands, secretory-excretory pore, vulva, anus and post-vulval uterine sac were affected by feeding on the fungi. However, male and female stylet length and V value showed the least variation in the studied populations
کلیدواژهها [English]
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